Role of Glutathione as a Signaling Molecule through the Overexpression of g-ECS in Transgenic Plant System

Ghanta, Srijani (2011) Role of Glutathione as a Signaling Molecule through the Overexpression of g-ECS in Transgenic Plant System. PhD thesis, Jadavpur University.

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    Supervisors

    SupervisorsEmail
    Chattopadhyay, Sharmila

    Abstract

    GSH, the so-called ‘master antioxidant’ is present in millimolar concentrations in the cells and due to this high concentration as well as the alteration in its content and status under various physiological conditions of the cells, numerous roles have been proposed as well as evidenced for this molecule in living organisms. In planta, a significant role of GSH in the cross-communication with other established signaling molecules to mitigate biotic stress (Fig. 1) has been proposed (Ghanta and Chattopadhyay 2011). In this investigation, genetic engineering approach has been used to develop transgenic Nicotiana tabacum with enhanced GSH content through the overexpression of g-ECS exhibiting resistance to biotrophic pathogen Pseudomonas syringae pv. tabaci. Further analysis of these transgenic lines at the transcript and protein levels have been performed to unravel the dynamic complexicity of plant disease resistance mechanism with special reference to the involvement of GSH in this process.

    Item Type: Thesis (PhD)
    URI: http://www.eprints.iicb.res.in/id/eprint/1
    Subjects: Drug Development/Diagnostics & Biotechnology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Madhan Muthu
    Date Deposited: 16 Sep 2011 11:00
    Last Modified: 20 Dec 2011 11:29
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