Effects of Arginine and Other Solution Additives on the Self-Association of Different Surfactants: An Investigation at Single-Molecule Resolution

Haldar, Shubhasis and Chattopadhyay, Krishnananda (2011) Effects of Arginine and Other Solution Additives on the Self-Association of Different Surfactants: An Investigation at Single-Molecule Resolution. Langmuir, 27 (10). pp. 5842-5849.

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    Abstract

    Fluorescence correlation spectroscopy is used to monitor the self-association of SDS and DTAB monomers at single-molecule resolution. Tetramethylrhodamine-5-maleimide (TMR) has been chosen as a probe because rhodamine dyes have been shown to bind surfactant micelles. Correlation functions obtained by FCS experiments have been fit using conventional discrete diffusional component analysis as well as the more recent maximum entropy method (MEM). Hydrodynamic radii calculated from the diffusion time values increase with surfactant concentration as the monomers self-associate. Effects of several solution additives on the self-association property of the surfactants have been studied. Urea and glycerol inhibit self-association, and arginine shows a dual nature. With SDS, arginine favors self-association, and with DTAB, it inhibits micelle formation. We propose surfactant self-association to be a “supersimplified” model of protein aggregation.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/105
    Subjects: Structural Biology & Bioinformatics
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Santanu Sadhukhan
    Date Deposited: 28 Sep 2011 17:52
    Last Modified: 03 Feb 2012 16:10
    Official URL: http://dx.doi.org/10.1021/la200580z
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