High-yielding Plasmid Extraction Method from Acidophilic Heterotrophic Bacteria of the Genus Acidiphilium

Samarendra K, Singh and Banerjee, Pataki C (2006) High-yielding Plasmid Extraction Method from Acidophilic Heterotrophic Bacteria of the Genus Acidiphilium. Analytical Biochemistry, 356 (2). pp. 229-234.

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    Abstract

    Plasmid yield from Acidiphilium strains always had been poor following various standard methods. We adopted some simple modiWcations in the alkaline lysis procedure to get a better yield of plasmid from these bacteria. An approximately 10- to 20-fold increase in the plasmid yield was achieved when harvested Acidiphilium cells were preincubated 16–20 h at pH 6 in nitrogen-free medium. Another independent approach showed that freezing (¡18 to ¡20 °C) of the harvested cells initially and at two subsequent steps in the alkaline lysis procedure of plasmid DNA extraction improved the yield further by 1.5- to 3-fold. The combination of these changes yielded at least 15-to 30-fold more plasmid from various Acidiphilium strains as compared with standard methods.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1084
    Subjects: Chemistry
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Santanu Sadhukhan
    Date Deposited: 28 Dec 2011 16:45
    Last Modified: 07 Feb 2012 11:40
    Official URL: http://dx.doi.org/10.1016/j.ab.2006.06.016
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