Synchronous Modulation of Cell Surface Lectin and Its Receptor in a Homologous Cell Population: A Novel Mechanism of Cellular Regulation

Banerjee, Saswti and Dungdung, Sandhya Rekha and Das, Kaushik and Majumder, Gopal Chandra (2006) Synchronous Modulation of Cell Surface Lectin and Its Receptor in a Homologous Cell Population: A Novel Mechanism of Cellular Regulation. Experimental Cell Research, 312 (12). pp. 2299-2308.

[img]

PDF
Restricted to IICB Scientists only

Download (518Kb) | Request a copy

    Abstract

    Testicular immotile sperm undergo maturation during epididymal transit when these cells pass through caput, corpus, and cauda-epididymal regions. Maturing goat spermatozoa specifically at the distal corpus epididymal stage show head-to-head autoagglutination when incubated in vitro in a modified Ringer's solution. Here, we show the biochemical mechanism of autoagglutination event and its functional significance. A lectin-like molecule located on sperm surface specifically interacts with its receptor of the neighboring homologous cells to cause autoagglutination. Lectin is a Ca++-dependent galactose-specific protein. Failure of the pre- and post-distal corpus sperm to show autoagglutination is due to lack of lectin-like molecule and its receptors, respectively. Maturing sperm at distal corpus stage acquire lectin-like molecule followed by sharp disappearance of its receptor, and this event is synchronously associated with the initiation of sperm forward motility that is essential for fertilization in vivo. Lectin and its receptor isolated from sperm plasma membrane showed high efficacy for blocking autoagglutination phenomenon. The data are consistent with the view that synchronous modulation of homologous cell surface lectin and their receptors constitutes a novel mechanism for cellular regulation by generating waves of signals by manipulating lectin–sugar-dependent “self-talk” and cell–cell “cross-talk”.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1088
    Subjects: Cell Biology & Physiology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Santanu Sadhukhan
    Date Deposited: 28 Dec 2011 17:02
    Last Modified: 27 Jan 2012 16:43
    Official URL: http://dx.doi.org/10.1016/j.yexcr.2006.03.020
    Links:

    Actions (login required)

    View Item