Cytotoxic and Apoptogenic Effect of Tea (Camellia sinensis Var. assamica) Root Extract (TRE) and Two of Its Steroidal Saponins TS1 and TS2 on Human Leukemic Cell Lines K562 and U937 and on cells of CML and ALL Patients

Ghosh, Papiya and Besra, Shila E and Tripathi, Gayatri and Mitra, Smita and Vedasiromoni, J R (2006) Cytotoxic and Apoptogenic Effect of Tea (Camellia sinensis Var. assamica) Root Extract (TRE) and Two of Its Steroidal Saponins TS1 and TS2 on Human Leukemic Cell Lines K562 and U937 and on cells of CML and ALL Patients. Leukemia Research, 30 (4). pp. 459-468.

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    Abstract

    The anticancer activity of di- and tri-terpenes and other polyphenolic compounds present in tea is already reported. We evaluated the cytotoxic and apoptogenic effect of tea root extract (TRE) and two of its steroidal saponins named as TS1 and TS2, on human cell lines and on cells from leukemic patients. It was found that TRE, TS1 and TS2 significantly decreased cell count and that TRE caused apoptosis, as confirmed morphologically by confocal microscopy and by flow-cytometric analysis using Annexin-V FITC and propidium iodide (PI). Cell count and MTT assay in normal white blood cells (WBC) of healthy volunteers revealed that TRE produced insignificant reduction in cell count and cytotoxicity.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1120
    Subjects: Drug Development/Diagnostics & Biotechnology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Santanu Sadhukhan
    Date Deposited: 29 Dec 2011 12:35
    Last Modified: 03 Feb 2012 11:49
    Official URL: http://dx.doi.org/10.1016/j.leukres.2005.08.018
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