Interaction of the Anticancer Plant Alkaloid Sanguinarine with Bovine Serum Albumin

Hossain, Maidul and Khan, Asma Yasmeen and Kumar, G Suresh (2011) Interaction of the Anticancer Plant Alkaloid Sanguinarine with Bovine Serum Albumin. PLOS ONE, 6 (4).

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    Abstract

    Background: Interaction of the iminium and alkanolamine forms of sanguinarine with bovine serum albumin (BSA) was characterized by spectroscopic and calorimetric techniques. Methodology/Principal Findings: Formation of strong complexes of BSA with both iminium and alkanolamine forms was revealed from fluorescence quenching of sanguinarine. Binding parameters calculated from Stern-Volmer quenching method revealed that the neutral alkanolamine had higher affinity to BSA compared to the charged iminium form. Specific binding distances of 3.37 and 2.38 nm between Trp 212 (donor) and iminium and alkanolamine forms (acceptor), respectively, were obtained from Forster resonance energy transfer studies. Competitive binding using the site markers warfarin and ibuprofen, having definite binding sites, demonstrated that both forms of sanguinarine bind to site I (subdomain IIA) on BSA. Sanguinarine binding alters protein conformation by reducing the a-helical organization and increasing the coiled structure, indicating a small but definitive partial unfolding of the protein. Thermodynamic parameters evaluated from isothermal titration calorimetry suggested that the binding was enthalpy driven for the iminium form but favoured by negative enthalpy and strong favourable entropy contributions for the alkanolamine form, revealing the involvement of different molecular forces in the complexation. Conclusions/Significance: The results suggest that the neutral alkanolamine form binds to the protein more favourably compared to the charged iminium, in stark contrast to the reported DNA binding preference of sanguinarine.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/123
    Subjects: Chemistry
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Santanu Sadhukhan
    Date Deposited: 29 Sep 2011 17:10
    Last Modified: 03 Feb 2012 16:27
    Official URL: http://dx.doi.org/10.1371/journal.pone.0018333
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