Nicotiana Tabacum Overexpressing c-ECS Exhibits Biotic Stress Tolerance likely through NPR1-Dependent Salicylic Acid-Mediated Pathway

Ghanta, Srijani and Bhattacharyya, Dipto and Sinha, Ragini and Banerjee, Anindita and Chattopadhyay, Sharmila (2011) Nicotiana Tabacum Overexpressing c-ECS Exhibits Biotic Stress Tolerance likely through NPR1-Dependent Salicylic Acid-Mediated Pathway. Planta, 235 (5). pp. 895-910.

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    Abstract

    The elaborate networks and the crosstalk of established signaling molecules like salicylic acid (SA), jasmonic acid (JA), ethylene (ET), abscisic acid (ABA), reactive oxygen species (ROS) and glutathione (GSH) play key role in plant defense response. To obtain further insight into the mechanism through which GSH is involved in this crosstalk to mitigate biotic stress, transgenic Nicotiana tabacum overexpressing Lycopersicon esculentum gammaglutamylcysteine synthetase (LeECS) gene (NtGB lines) were generated with enhanced level of GSH in comparison with wild-type plants exhibiting resistance to pathogenesis as well. The expression levels of non-expressor of pathogenesis- related genes 1 (NPR1)-dependent genes like pathogenesis-related gene 1 (NtPR1), mitogen-activated protein kinase kinase (NtMAPKK), glutamine synthetase (NtGLS) were significantly enhanced alongwith NtNPR1. However, the expression levels of NPR1-independent genes like NtPR2, NtPR5 and short-chain dehydrogenase/ reductase family protein (NtSDRLP) were either insignificant or were downregulated. Additionally, increase in expression of thioredoxin (NtTRXh), S-nitrosoglutathione reductase 1 (NtGSNOR1) and suppression of isochorismate synthase 1 (NtICS1) was noted. Comprehensive analysis of GSH-fed tobacco BY2 cell line in a time-dependent manner reciprocated the in planta results. Better tolerance of NtGB lines against biotrophic Pseudomonas syringae pv. tabaci was noted as compared to necrotrophic Alternaria alternata. Through two-dimensional gel electrophoresis (2-DE) and image analysis, 48 differentially expressed spots were identified and through identification as well as functional categorization, ten proteins were found to be SA-related. Collectively, our results suggest GSH to be a member in cross-communication with other signaling molecules in mitigating biotic stress likely through NPR1- dependent SA-mediated pathway.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/124
    Subjects: Drug Development/Diagnostics & Biotechnology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Santanu Sadhukhan
    Date Deposited: 29 Sep 2011 17:20
    Last Modified: 31 Jan 2012 12:47
    Official URL: http://dx.doi.org/10.1007/s00425-011-1349-4
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