Potent Spermicidal Effect of Oleanolic acid 3-Beta-D-gGlucuronide, an Active Principle Isolated from the Plant Sesbania Sesban Merrill.

Das, Nilanjana and Chandran, Poornima and Chakraborty, Smritinath (2011) Potent Spermicidal Effect of Oleanolic acid 3-Beta-D-gGlucuronide, an Active Principle Isolated from the Plant Sesbania Sesban Merrill. Contraception, 83 (2). pp. 167-175.

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    Abstract

    The spermicidal activity of oleanolic acid 3-β-D-glucuronide (OAG), an active principle isolated from root extracts of Sesbania sesban, was evaluated. Under the Sander–Cramer test criteria, the sperm-immobilizing activity of OAG was studied using highly motile rat sperm. Sperm mortality and membrane integrity were assessed by supravital staining, hypo-osmotic swelling (HOS), transmission electron microscopy (TEM) and sperm membrane lipid peroxidation (LPO). In vitro microbicidal potential and hemolytic index of OAG were examined in Lactobacillus culture and rat red blood corpuscles (RBCs), respectively. Post-intravaginal OAG application, the in vivo contraceptive efficacy was evaluated in rats. Ames test determined the carcinogenic potential of OAG. The minimum effective concentration (MEC) of OAG was 50 mcg/mL. More than 97% of the OAG-treated sperm lost their HOS responsiveness in a dose-dependent manner. TEM and LPO revealed that OAG affected the sperm membrane integrity. OAG declined fertility to zero, was nonmutagenic and was not harmful to lactobacillus. OAG has significant spermicidal activity that may be explored further.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1246
    Subjects: Cell Biology & Physiology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 18 Jan 2012 12:14
    Last Modified: 18 Jan 2012 12:14
    Official URL: http://dx.doi.org/10.1016/j.contraception.2010.05....
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