Manganese and Iron both Influence the Shoot Transcriptome of Typha Angustifolia Despite Distinct Preference towards Manganese Accumulation

Chakraborty, D and Kumar, S Abhay and Sen, M and Apte, S K and Das, S and Acharya, R and Das, T and Reddy, A V R and Roychoudhury, Susanta and Rajaram, H and Seal, A (2011) Manganese and Iron both Influence the Shoot Transcriptome of Typha Angustifolia Despite Distinct Preference towards Manganese Accumulation. Plant Soil, 342. pp. 301-317.

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    Abstract

    Typha angustifolia is a metal hypertolerant grass that predominates the wetlands of uranium tailings in Jaduguda, India, contaminated with extreme levels of iron (Fe) and manganese (Mn). In the paper investigations were carried out to understand the molecular mechanism of metal tolerance in this tolerant macrophyte. Metal analysis was coupled with fluorescent differential display (FDD) and reverse northern to compare the metal profile and transcriptome between Typha plants growing in the tailings versus a control wetland. The expressions of the validated transcripts in Mn and Fe stress were confirmed using reverse northern and semiquantitaive RT-PCR. Typha selectively accumulated and stored Mn in shoots but excluded/restricted Fe both in the field and in vitro. Differential expressions of 23 ESTs were validated among 118 FDD genes. Although Typha behaved as a Mn accumulator, these transcripts were found to be regulated in a complex fashion not only by Mn accumulated in the shoots but also by Fe excluded from the shoots. Our results provide the first report in the molecular understanding of metal tolerance in Typha angustifolia and indicate a

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/127
    Subjects: Molecular & Human Genetics
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Shyamal Nath
    Date Deposited: 29 Sep 2011 19:21
    Last Modified: 22 Jul 2015 12:28
    Official URL: http://dx.doi.org/10.1007/s11104-010-0696-4
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