UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay

Banerjee, Nupur and Bhattacharyya, Debasish (2011) UDP-Glucose Dehydrogenase from Capra Hircus Liver: Purification, Partial Characterization and Evaluation as a Coupling Enzyme in UDP-Galactose 4-Epimerase Assay. Journal of Molecular Catalysis B: Enzymatic, 68 (1). pp. 37-43.

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    Abstract

    UDP-glucose dehydrogenase from Capra hircus has been purified to homogeneity by salt fractionations, heat treatment and chromatographic steps. It is a homohexamer of about 300 kDa. Though the basic physical and enzymatic properties of the caprine enzyme are comparable to those of the beef liver enzyme, it has lower energy of activation and different entropy and enthalpy for the transition state during catalysis. The caprine enzyme can act suitably as an auxiliary enzyme in the coupled assay system for UDP-galactose 4-epimerase

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1296
    Subjects: Structural Biology & Bioinformatics
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 20 Jan 2012 13:28
    Last Modified: 20 Jan 2012 13:28
    Official URL: http://dx.doi.org/10.1016/j.molcatb.2010.09.007
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