Role of cAMP Signaling in the Survival and Infectivity of the Protozoan Parasite, Leishmania donovani

Biswas, Arumina and Bhattacharya, Arijit and Das, Pijush K (2011) Role of cAMP Signaling in the Survival and Infectivity of the Protozoan Parasite, Leishmania donovani. Molecular Biology International . pp. 1-9.

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    Abstract

    Leishmania donovani, while invading macrophages, encounters striking shift in temperature and pH (from 22◦ C and pH 7.2 to 37◦ C and pH 5.5), which act as the key environmental trigger for differentiation, and increases cAMP level and cAMP-mediated responses. For comprehensive understanding of cAMP signaling, we studied the enzymes related to cAMP metabolism. A stage-specific and developmentally regulated isoform of receptor adenylate cyclase (LdRACA) showed to regulate differentiation-coupled induction of cAMP. The soluble acidocalcisomal pyrophosphatase, Ldvsp1, was the major isoform regulating cAMP level in association with LdRACA. A differentially expressed soluble cytosolic cAMP phosphodiesterase (LdPDEA) might be related to infection establishment by shifting trypanothione pool utilization bias toward antioxidant defense. We identified and cloned a functional cAMP-binding effector molecule from L. donovani (a regulatory subunit of cAMP-dependent protein kinase, LdPKAR)that may modulate metacyclogenesis through induction of autophagy. This study reveals the significance of cAMP signaling in parasite survival and infectivity.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1329
    Subjects: Infectious Diseases and Immunology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Dr. Arunima Biswas
    Date Deposited: 17 Jul 2012 17:11
    Last Modified: 17 Jul 2012 17:11
    Official URL: http://dx.doi.org/10.4061/2011/782971
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