Complete Nucleotide Sequence of a Quinolone Resistance Gene (qnrS2) Carrying Plasmid of Aeromonas Hydrophila Isolated from Fish.

Majumdar, Tanmay and Das, Bhabatosh and Bhadra, Rupak K and Damc, Bomba and Mazumder, Shibnath (2011) Complete Nucleotide Sequence of a Quinolone Resistance Gene (qnrS2) Carrying Plasmid of Aeromonas Hydrophila Isolated from Fish. Plasmid, 66 (2). pp. 79-84.

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    Abstract

    Aeromonas hydrophila strain AO1 isolated from an infected fish was found to be resistant to several quinolones. A plasmid isolated from the strain AO1, termed pBRST7.6, was cloned and sequenced and shown to be 7621 bp in length with a GC content of 60%. Further analysis confirmed that it contained a gene with 100% identity to qnrS2 genes described in plasmids associated with other Aeromonas species, the product of which usually confers increased resistance to quinolones. The plasmid backbone contained a replication initiation module (repA repC) belonging to the IncQ-family and two genes (mobC and mobB), the products of which are putatively involved in plasmid mobilization. Putative iteron-based origin of replication and characteristic oriT like sequences were also present in the plasmid. The result suggests that Aeromonas spp. carrying plasmids with quinolone resistance genes are potential reservoirs of antimicrobial resistance determinants in the environment.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/1333
    Subjects: Infectious Diseases and Immunology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 31 Jan 2012 13:01
    Last Modified: 31 Jan 2012 13:01
    Official URL: http://dx.doi.org/10.1016/j.plasmid.2011.05.001
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