A Smart Molecule for Selective Sensing of Nitric Oxide: Conversion of NO to HSNO; Relevance of Biological HSNO Formation

Islam, Abu Saleh Musha and Bhowmick, Rahul and Pal, Kaberi and Katarkar, Atul and Chaudhuri, Keya and Ali, Mahammad (2017) A Smart Molecule for Selective Sensing of Nitric Oxide: Conversion of NO to HSNO; Relevance of Biological HSNO Formation. Inorganic Chemistry, 56. pp. 4324-4331. ISSN 2017, 56, 4324−4331

[img]

PDF
Restricted to IICB Scientists only

Download (1992Kb) | Request a copy

    Abstract

    A smart molecule, QT490, containing thiosemicarbazide moiety acts as a highly selective turn-on in vitro NO sensor through the unprecedented NO-induced transformation of thiosemicarbazide moiety to 1,3,4-oxadiazole heterocycle with the concomitant release of HSNO, thereby eliminating any interference from various endogenous biomolecules including dehydroascorbic acid, ascorbic acid, etc. The kinetic studies of the reactions between QT490 and NO provide a mechanistic insight into formation of HSNO/RSNO from the reaction between H2S/RSH and NO in the biological system. This novel probe is non-cytotoxic, cell permeable, water-soluble, and appropriate for intracellular cytoplasmic NO sensing with the possibilities of in vivo applications.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/2664
    Subjects: Molecular & Human Genetics
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 02 Jun 2017 11:16
    Last Modified: 02 Jun 2017 11:16
    Official URL: http://dx.doi.org/10.1021/acs.inorgchem.6b02787
    Links:

    Actions (login required)

    View Item