Photo-induced cytotoxicity and anti-metastatic activity of ruthenium(II)–polypyridyl complexes functionalized with tyrosine or tryptophan

Ramu, Vadde and Aute, Sunil and Taye, Nandaraj and Guha, Rweetuparna and Walker, Michael G and Mogare, Devaraj and Parulekar, Apoorva and Thomas, Jim A and Chattopadhyay, Samit and Das, Amitava (2017) Photo-induced cytotoxicity and anti-metastatic activity of ruthenium(II)–polypyridyl complexes functionalized with tyrosine or tryptophan. Dalton Transactions, 46. pp. 6634-6644.

[img]

PDF
Restricted to IICB Scientists only

Download (2366Kb) | Request a copy

    Abstract

    The synergistic effect of oxygen, light, and photosensitizer (PS) has found applications in medicine for the treatment of cancer through photodynamic therapy (PDT). Induction of apoptosis to cancerous cells will prevent tumor metastasis that spreads cancer cells to the neighboring organs/tissues. Herein, we report the two apoptotic Ru(II)–polypyridyl complexes that are functionalized with pendant amino acid moieties tyrosine (1) and tryptophan (2), respectively. These two water soluble complexes were found to interact strongly (K1a = (1.18 ± 0.28) × 105 M−1 and K2a = (1.57 ± 0.77) × 105 M−1) with CT-DNA. Isothermal titration calorimetry (ITC) studies revealed that these complexes bind to CT-DNA through an entropically driven process. Both the complexes showed photo-induced cytotoxicity and exhibit apoptotic activity under photo-irradiation conditions. The comet assay indicated that these complexes can damage cellular DNA, which is attributed to the significant build-up of 1O2 level even on irradiation with low intensity light (10 J cm−2, λRange 450–480 nm). This photoinduced DNA damage and apoptosis in A549 cells was induced by reactive oxygen species (ROS) and occurred through up-regulation of apoptotic marker caspase-3. Control experiments under dark conditions revealed an insignificant cytotoxicity towards these cells for two photosensitive molecules

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/2687
    Subjects: Cancer Biology and Inflammatory Disorder Division
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 21 Jun 2017 13:25
    Last Modified: 21 Jun 2017 13:25
    Links:

    Actions (login required)

    View Item