Physical chemistry in a single live cell: confocal microscopy

Amin, Md. Asif and Nandi, Somen and Mondal, Prasenjit and Mahata, Tanushree and Ghosh, Surajit and Bhattacharyya, Kankan (2017) Physical chemistry in a single live cell: confocal microscopy. Phys. Chem. Chem. Phys, 19 (20). pp. 12620-12627.


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    A live cell is a complex, yet extremely important container. Understanding the dynamics in a selected intracellular component is a challenging task. We have recently made significant progress in this direction using a confocal microscope as a tool. The smallest size of the focused spot in a confocal microscope is B0.2 mm (200 nm). This is nearly one hundred times smaller than the size of a live cell. Thus, one can selectively study different intracellular components/organelles in a live cell. In this paper, we discuss how one can image different intracellular components/organelles, record fluorescence spectra and decay at different locations, ascertain local polarity and viscosity, and monitor the dynamics of solvation, proton transfer, red-ox and other phenomena at specified locations/organelles inside a cell. We will highlight how this knowledge enriched us in differentiating between cancer and non-cancer cells, 3D tumor spheroids and towards drug delivery

    Item Type: Article
    Subjects: Chemistry
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 08 Aug 2017 12:34
    Last Modified: 08 Aug 2017 12:34
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