Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors

Mukherjee, Sumana and Chowdhury, Sudeshna and Ghorai, Shakuntala and Pal, Swagata and Khowala, Suman (2006) Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors. Biotechnol Lett, 28 (21). pp. 1773-1778.

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    Abstract

    In presence of the glycosylation inhibitors, 2-deoxy-D-glucose (1 mg/ml), tunicamycin (30 lg/ml), 1-deoxynojirimycin (30 lg/ml) and D-glucono-d-lactone (1 mg/ml), total cellobiase activity, in the extracellular, intracellular and cell bound fractions, of the fungus Termitomyces clypeatus grown in 20 ml cellobiose medium (1%, w/v) increased by 50-, 1.8-, 2.4-, 1.3-fold, respectively, with respect to control medium (16.3 U). The inhibitors also stimulated secretion of 95% of the total protein in culture medium, except D-glucono-d-lactone which released 60% of the total protein. 2-Deoxy-D-glucose (1 mg/ml) led to production of extracellular cellobiase up to 40 U/ml, whereas in absence of the inhibitors only 0.59 U/ml enzyme was detected.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/424
    Subjects: Chemistry
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Shyamal Nath
    Date Deposited: 01 Nov 2011 17:36
    Last Modified: 03 Feb 2012 12:40
    Official URL: htpp://dx.doi.org/10.1007/s10529-006-9150-3
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