Calcium-dependent proteolytic activity of a cysteine protease caldonopain is detected during Leishmania infection

Dey, Runu and Bhattacharya, Jharna and Datta, Salil C (2006) Calcium-dependent proteolytic activity of a cysteine protease caldonopain is detected during Leishmania infection. Molecular and Cellular Biochemistry, 281 (1-2). pp. 27-33.

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    Abstract

    A calcium-activated protease caldonopain in the cytosolic fraction of Leishmania donovani has been found to digest different endogenous proteins when subjected to SDS-PAGE. Gelatin-embedded gel electrophoresis confirms presence of calciumdependent protease activity. Ca2+ affects proteolytic activity after 10 h. When host–parasite interaction was conducted in vitro, caldonopain was found to be active after 10 h of incubation with calcium. A 67-kDa protein is specifically digested during this time and two new proteins of 45 and 36 kDa appeared in SDS-PAGE electrophoregram. This belated action of calcium towards protease activity may be pre-requisite to facilitate invasion of host tissues and thereby mediate protein metabolism during survival of this pathogen both independently and intracellularly. It is likely that calcium metabolism in promastigotes and amastigotes does not propagate in the same manner. Involvement of calcium to initiate caldonopain activity may be critically associated with signal transduction pathways which may be responsible for the pathobiological action of this parasite. We propose that caldonopain could be a potential target to develop new chemotherapeutic approach against leishmaniasis.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/426
    Subjects: Drug Development/Diagnostics & Biotechnology
    Infectious Diseases and Immunology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 01 Nov 2011 13:19
    Last Modified: 03 Feb 2012 11:45
    Official URL: http://dx.doi.org/ 10.1007/s11010-006-0171-y
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