Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal

Sah, Malay and Jaisankar , P and Das, Satadal and Sarkar, Kalyan K and Roy, Soma and Besra, Shila E and Vedasiromani, J R and Ghosh, Debashish and Sana, Barindra and Mukherjee, Joydeep (2006) Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal. Biotechnol Lett, 28 (14). pp. 1083-1088.

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    Abstract

    Four marine actinobacteria tolerant to 200 g NaCl l)1 were screened for antibacterial activity against eight patient-derived multiple drug resistant (MDR) bacteria. The active compound (MW 300.2, predicted molecular formula C20H28O2) from an actinobacterium, was inhibitory to three Gram-positive and three Gramnegative MDR bacteria, seven non-clinical Gram-positive, four Gram-negative bacteria and five fungi (MIC: 3.5–4.0 lg ml)1). Also, 54% of human leukemia (HL-60) cells were killed by the compound at 0.05 lg ml)1. Bioreactor production demonstrated unusual primary metabolite kinetics. Molecular phylogenetic analysis showed this typical intertidal inhabitant to be a member of the Streptomyces genus and distinct from other salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic databases, our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/428
    Subjects: Drug Development/Diagnostics & Biotechnology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Shyamal Nath
    Date Deposited: 01 Nov 2011 17:36
    Last Modified: 07 Feb 2012 11:28
    Official URL: htpp://dx.doi.org/10.1007/s10529-006-9054-2
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