Mapping the Antigenicity of the Parasites in Leishmania donovani Infection by Proteome Serology

Forgber, Michael and Basu, Rajatava and Roychoudhury, Kaushik and Theinert, Stephan M and Roy, Syamal and Sundar, Shyam and Walden, Peter (2006) Mapping the Antigenicity of the Parasites in Leishmania donovani Infection by Proteome Serology. PLOS One, 1 (1).

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    Abstract

    Leishmaniasis defines a cluster of protozoal diseases with diverse clinical manifestations. The visceral form caused by Leishmania donovani is the most severe. So far, no vaccines exist for visceral leishmaniasis despite indications of naturally developing immunity, and sensitive immunodiagnostics are still at early stages of development. Methodology/ Principle Findings. Establishing a proteome-serological methodology, we mapped the antigenicity of the parasites and the specificities of the immune responses in human leishmaniasis. Using 2-dimensional Western blot analyses with sera and parasites isolated from patients in India, we detected immune responses with widely divergent specificities for up to 330 different leishmanial antigens. 68 antigens were assigned to proteins in silver- and fluorochrome-stained gels. The antigenicity of these proteins did not correlate with the expression levels of the proteins. Although some antigens are shared among different parasite isolates, there are extensive differences and no immunodominant antigens, but indications of antigenic drift in the parasites. Six antigens were identified by mass spectrometry. Conclusions/Significance. Proteomics-based dissection of the serospecificities of leishmaniasis patients provides a comprehensive inventory of the complexity and interindividual heterogeneity of the host-responses to and variations in the antigenicity of the Leishmania parasites. This information can be instrumental in the development of vaccines and new immune monitoring and diagnostic devices

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/475
    Subjects: Infectious Diseases and Immunology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Ms Sutapa Ganguly
    Date Deposited: 02 Nov 2011 11:46
    Last Modified: 18 Jul 2012 15:17
    Official URL: http://dx.doi.org/10.1371/journal.pone.0000040
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