Curcumin Alleviates Matrix Metalloproteinase-3 and -9 Activities during Eradication of Helicobacter pylori Infection in Cultured Cells and Mice

Kundu, Parag and De, Ronita and Pal, Ipsita and Mukhopadhyay, Asish K and Saha, Dhira Rani and Swarnakar, Snehasikta (2011) Curcumin Alleviates Matrix Metalloproteinase-3 and -9 Activities during Eradication of Helicobacter pylori Infection in Cultured Cells and Mice. PLoS ONE, 6 (1).

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    Abstract

    Current therapy-regimens against Helicobacter pylori (Hp) infections have considerable failure rates and adverse side effects that urge the quest for an effective alternative therapy. We have shown that curcumin is capable of eradicating Hp-infection in mice. Here we examine the mechanism by which curcumin protects Hp infection in cultured cells and mice. Since, MMP-3 and -9 are inflammatory molecules associated to the pathogenesis of Hp-infection, we investigated the role of curcumin on inflammatory MMPs as well as proinflammatory molecules. Curcumin dose dependently suppressed MMP-3 and -9 expression in Hp infected human gastric epithelial (AGS) cells. Consistently, Hp-eradication by curcumin-therapy involved significant downregulation of MMP-3 and -9 activities and expression in both cytotoxic associated gene (cag)+ve and cag-ve Hp-infected mouse gastric tissues. Moreover, we demonstrate that the conventional triple therapy (TT) alleviated MMP-3 and -9 activities less efficiently than curcumin and curcumin’s action on MMPs was linked to decreased pro-inflammatory molecules and activator protein-1 activation in Hp-infected gastric tissues. Although both curcumin and TT were associated with MMP-3 and -9 downregulation during Hp-eradication, but unlike TT, curcumin enhanced peroxisome proliferatoractivated receptor-c and inhibitor of kappa B-a. These data indicate that curcumin-mediated healing of Hp-infection involves regulation of MMP-3 and -9 activities.

    Item Type: Article
    URI: http://www.eprints.iicb.res.in/id/eprint/68
    Subjects: Drug Development/Diagnostics & Biotechnology
    Divisions: Indian Institute of Chemical Biology
    Depositing User: Mr Shyamal Nath
    Date Deposited: 28 Sep 2011 19:38
    Last Modified: 31 Jan 2012 13:57
    Official URL: http://dx.doi.org/10.1371/journal.pone.0016306.g00...
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